Reconstituting peptides is one of the first skills any researcher needs to master. Lyophilized (freeze-dried) peptides arrive as a powder and must be mixed with bacteriostatic water (BAC water) before use. This guide walks through the process step by step.
What You Need
Before reconstituting, gather the following: your peptide vial, bacteriostatic water (BAC water), an alcohol prep pad, a 3ml syringe with needle, and your insulin syringe for drawing doses.
Step 1 — Wipe the Tops
Use an alcohol prep pad to wipe the rubber stopper on both your peptide vial and your BAC water vial. Allow them to air dry for 10–15 seconds before proceeding.
Step 2 — Draw BAC Water
Draw your desired amount of BAC water into the 3ml syringe. The amount you use determines your concentration. A common starting point for most peptides is 1–2mL of BAC water per vial.
Step 3 — Add Water Slowly
Insert the needle into the peptide vial and angle it so the BAC water runs down the inside wall of the vial rather than directly onto the powder. Add the water slowly. Do not shake the vial — swirl gently until the powder fully dissolves.
Step 4 — Calculate Your Concentration
Use the formula: Concentration (mcg/mL) = Vial size in mcg ÷ mL of BAC water added. For example, a 5mg (5000mcg) vial with 2mL of BAC water = 2500mcg/mL concentration.
Step 5 — Draw Your Dose
Use our Reconstitution Calculator to determine exactly how many units to draw on your U-100 insulin syringe for your target dose.
Storage
Store reconstituted peptides in the refrigerator. Most reconstituted peptides remain stable for 4–6 weeks when refrigerated. Do not freeze reconstituted peptides.
All products sold at EhBuddy Peptides are for laboratory and scientific research purposes only. This guide is provided for informational purposes and is not intended as medical advice.
